Normalisation / Affymetrix gene arrays

Description

Preprocesses Affymetrix gene array CEL-files to give expression estimates for genes.

Parameters

Chiptype (empty, Human, Mouse, Rat) [empty]

Details

Calculates expression estimates using the RMA normalization method and performs log2-transformation. Note that Affymetrix detection calls are not available because there are no mismatch probes.

It is a known problem that a sizeable part of the probes on Affymetrix expression arrays map to wrong genes. In order to circumvent this problem, Chipster uses custom CDF files from the University of Michigan, which remap the probes using current knowledge.

Output

A tab-delimited text file containing gene names, gene symbols and expression estimates.