Given a fasta file of aligned 16S rRNA sequences and a groups file generated by Mothur, this tool assigns sequences to taxonomic units (~ species). In addition to classification, it generates a count table and a phenodata file, which you can use to assign samples to different experimental groups for further statistical analysis.
Assigns aligned 16S rRNA sequences to taxonomic units using the Wang method and the Silva reference set and its taxonomy file. The bootstrap confidence score is calculated with 1000 iterations.
This tool is based on the Classify.seqs command of the Mothur package.
The analysis output consists of the following:
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol, 2009. 75(23):7537-41.