BWA MEM for paired-end end reads

Description

This tool aligns paired-end reads to the reference genome sequence given by the user. The alignment is constructed using the BWA MEM algorithm. The genome needs to be supplied in fasta format and the reads in two fastq files.

  • More information: BWA manual
  • Parameters

    By default, the tool runs the mapping using the default parameters of BWA MEM algorithm. These parameters are intended for, Illumina, 454 or IonTorrent reads files where the length of the reads is more than 70 base pairs. If your data is from PacBio sequencer, you should use the Data source: PacBio subreads setting to switch to parameters suitable for PacBio reads.

    Output

    As a result the tool returns a sorted and indexed BAM-formatted alignment, which is ready for viewing in the Chipster genome browser.