Displays restriction enzyme binding sites in a nucleotide sequence.
The cleavage sites are determined with EMBOSS command remap that scans one or more nucleotide sequences for recognition sites and/or cut sites for a supplied set of restriction enzymes. One or more restriction enzymes can be specified or alternatively all the enzymes in the REBASE database can be investigated. The minimum length of a recognition site to be reported must be specified. It writes an output file showing the location of the cut sites and (optionally) the recognition sites. Sites on both strands are shown by default but there are many options to control exactly what sites are reported and the format of the output file. Optionally, the translated sequence is reported. Additionally, the output file lists enzymes that cut / do not cut the sequence and which match / do not matching certain specified criteria.
The input sequences can be in any of the EMBOSS compatible sequence formats.
For more details, please check the manual page of remap command.
Output is a html formatted file.