Digital expression
Description
This Drop-seq tool extracts the digital gene expression data from an aligned library.
There are two outputs available:
-the primary is the DGE matrix, with each a row for each gene, and a column for each cell
-the secondary analysis is a summary of the DGE matrix on a per cell level,
indicating the number of genes and transcripts observed.
The selection of the sets of cells
- Number of barcodes: Counts the number of reads per cell barcode and only includes cells that
have at least this many reads.
- Number of genes per cell: Select cells that have at least this many genes.
For more details, please check the Drop-seq manual.
Parameters
- Number of barcodes [100]
- Number of genes per cell [100]
Output
- digital_expression.tsv: The DGE matrix (primary output)
- digital_expression_summary.txt: summary of the DGE matrix on a per cell level (the secondary output)